Standardization of Cystatin C: development of primary and secondary reference preparations.
Författare
Summary, in English
A Primary Reference Preparation has been produced using pure, recombinant, Cystatin C in a solvent of 0.1 mol/L KCl. Dry mass determination of the Primary Reference Preparation resulted in a Cystatin C concentration of 5.20 g/L. Agarose-electrophoresis and SDS-electrophoresis, as well as N-terminal sequencing, verified the purity, homogeneity and identity of Cystatin C in the Primary Reference Preparation. For the Secondary Reference Preparation, a serum pool was collected and stabilized. A pilot batch was made to verify the selected procedure and spiking with the pure, recombinant Cystatin C. The final Secondary Reference Preparation is now produced (4468 vials) and ready for value assignment and further characterization.
Avdelning/ar
Publiceringsår
2008
Språk
Engelska
Sidor
67-70
Publikation/Tidskrift/Serie
Scandinavian Journal of Clinical & Laboratory Investigation
Volym
68
Issue
s241
Länkar
Dokumenttyp
Artikel i tidskrift
Förlag
Informa Healthcare
Ämne
- Medicinal Chemistry
- Pharmacology and Toxicology
Nyckelord
- Primary Reference Preparation
- Secondary Reference Preparation
- standardization of Cystatin C
- dry mass determination
- Cystatin C
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 1502-7686