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C-13 incorporation into signature fatty acids as an assay for carbon allocation in arbuscular mycorrhiza

In English

Författare

Summary, in English

The ubiquitous arbuscular mycorrhizal fungi consume significant amounts of plant assimilated C, but this C flow has been difficult to quantify. The neutral lipid fatty acid 16:1ω 5 is a quantitative signature for most arbuscular mycorrhizal fungi in roots and soil. We measured carbon transfer from four plant species to the arbuscular mycorrhizal fungus Glomus intraradices by estimating C-13 enrichment of 16:1ω 5 and compared it with C-13 enrichment of total root and mycelial C. Carbon allocation to mycelia was detected within 1 day in monoxenic arbuscular mycorrhizal root cultures labeled with [C-13] glucose. The 13 C enrichment of neutral lipid fatty acid 16:1ω 5 extracted from roots increased from 0.14% 1 day after labeling to 2.2% 7 days after labeling. The colonized roots usually were more enriched for C-13 in the arbuscular mycorrhizal fungal neutral lipid fatty acid 16:1w5 than for the root specific neutral lipid fatty acid 18:2ω 6,9. We labeled plant assimilates by using (CO2)-C-13 in whole-plant experiments. The extraradical mycelium often was more enriched for C-13 than was the intraradical mycelium, suggesting rapid translocation of carbon to and more active growth by the extraradical mycelium. Since there was a good correlation between 13C enrichment in neutral lipid fatty acid 16:1ω 5 and total C-13 in extraradical mycelia in different systems (r(2) = 0.94), we propose that the total amount of labeled C in intraradical and extraradical mycelium can be calculated from the C-13 enrichment of 16:1ω 5. The method described enables evaluation of C flow from plants to arbuscular mycorrhizal fungi to be made without extraction, purification and identification of fungal mycelia.

Publiceringsår

2005

Språk

Engelska

Sidor

2592-2599

Publikation/Tidskrift/Serie

Applied and Environmental Microbiology

Volym

71

Issue

5

Dokumenttyp

Artikel i tidskrift

Förlag

American Society for Microbiology

Ämne

  • Biological Sciences
  • Ecology

Status

Published

Forskningsgrupp

  • Microbial Ecology
  • Plant Biology

ISBN/ISSN/Övrigt

  • ISSN: 0099-2240