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Identification of the molecular and genetic basis of PX2, a glycosphingolipid blood group antigen lacking on globoside-deficient erythrocytes.

In English

Författare

Summary, in English

The x2 glycosphingolipid is expressed on erythrocytes from individuals of all common blood group phenotypes and elevated on cells of the rare P/P1/P(k)-negative p blood group phenotype. Globoside or P antigen is synthesized by UDP-N-acetylgalactosamine:globotriaosylceramide 3-β-N-acetylgalactosaminyltransferase encoded by B3GALNT1. It is the most abundant non-acid glycosphingolipid on erythrocytes and displays the same terminal disaccharide, GalNAcβ3Gal, as x2. We encountered a patient with mutations in B3GALNT1 causing the rare P-deficient P1 (k) phenotype and whose pre-transfusion plasma was unexpectedly incompatible with p erythrocytes. The same phenomenon was also noted in seven other unrelated P-deficient individuals. Thin-layer chromatography, mass spectrometry and flow cytometry was used to show that the naturally-occurring antibodies made by p individuals recognise x2 and sialylated forms of x2, while x2 is lacking on P-deficient erythrocytes. Overexpression of B3GALNT1 resulted in synthesis of both P and x2. Knockdown experiments with siRNA against B3GALNT1 diminished x2 levels. We conclude that x2 fulfills blood group criteria and is synthesized by β1,3GalNAc-T1. Based on this linkage, we proposed that x2 joins P in the GLOB blood group system (ISBT 028) and is renamed PX2 (GLOB2). Thus, in the absence of a functional P synthase neither P nor PX2 are formed. As a consequence, naturally-occurring anti-P and anti-PX2 can be made. Until the clinical significance of anti-PX2 is known, we also recommend that rare P1 (k) or P2 (k) RBC units are preferentially selected for transfusion to P(k) patients since p RBCs may pose a risk for hemolytic transfusion reactions due to their elevated PX2 levels.

Publiceringsår

2015

Språk

Engelska

Sidor

18505-18518

Publikation/Tidskrift/Serie

Journal of Biological Chemistry

Volym

290

Issue

30

Dokumenttyp

Artikel i tidskrift

Förlag

American Society for Biochemistry and Molecular Biology

Ämne

  • Hematology

Status

Published

Forskningsgrupp

  • Transfusion Medicine

ISBN/ISSN/Övrigt

  • ISSN: 1083-351X