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Enzyme conformational alterations detected by partition column chromatography

In English

Författare

  • Christer Wingren
  • Ulla-Britt Hansson

Summary, in English

In this paper, we demonstrate the ability of liquid-liquid partition chromatography (LLPC) to detect conformational alterations occurring in well-characterized enzymes. The conformational changes induced in dehydrogenases such as alcohol dehydrogenase (ADH), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), lactate dehydrogenases (LDH) and malate dehydrogenase (MDH) upon binding of ligand(s) were detectable by LLPC. The ligand-dependent equilibrium between two forms of citrate synthase (CS), glutamate-oxaloacetate transaminase (GOT), hexokinase (HK) and 3-phosphoglycerate kinase (PGK) could also be demonstrated. Furthermore, different conformational forms of some of the apoenzymes could also be detected and separated by LLPC. The results obtained here are discussed in relation to those obtained by other methods.

Publiceringsår

1996

Språk

Engelska

Sidor

113-122

Publikation/Tidskrift/Serie

Journal of Chromatography. B

Volym

680

Issue

1-2

Dokumenttyp

Artikel i tidskrift

Förlag

Elsevier

Ämne

  • Biological Sciences

Nyckelord

  • Partition column chromatography
  • Conformational changes
  • Enzymes

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1572-6495