Binding of extracellular matrix molecules by staphylococci from wild herbivores

Summary, in English

Nine strains of staphylococci were examined for their antibiotic resistance and for binding of seven extracellular matrix (ECM) molecules (bovine mucin, porcine mucin, porcine fibronectin, bovine fibrinogen, fetuin, bovine lactoferrin and heparin) immobilized on latex beads in the particle agglutination assay. All nine strains were resistant to bacitracin, and most of them were multiresistant. Four strains displayed resistance to 5 of 13 antibiotics tested. Different binding capability between individual strains was observed. There were strains binding several ECM molecules (as S. warneri SW6 and S.. epidermidis SE14) as well as strains which bound only one (S. aureus SA 11) or no ECM molecule (S. saprophyticus SS16) of seven tested. While some ECM molecules, e.g. porcine fibronectin and heparin, were bound by most of strains, others (e.g. mucins) were bound only by I or 2 strains. Some strains bound these substrates weakly, however, other strains displayed strong binding especially of heparin and porcine fibronectin. The preincubation of bacteria for I hour at room temperature with a protein used subsequently in the PAA completely prevented the agglutination reaction, thus indicating the specificity of the assay.

• Författare: I Styriak; A Laukova; Åsa Ljungh
• Avdelning/ar: Avdelningen för medicinsk mikrobiologi
• Publiceringsår: 2002
• Språk: Engelska
• Sidor: 369-374
• Publikation/Tidskrift/Serie: Acta Veterinaria Brno
• Volym: 71
• Issue: 3
• Länkar: http://vfu-www.vfu.cz/acta-vet/vol71/pdf/71_369.pdf
• Dokumenttyp: Artikel i tidskrift
• Förlag: VYSOKA SKOLA VETERINARNI FARMACEUTICKA
• Ämne: Microbiology in the medical area
• Nyckelord: heparin; fetuin; fibrinogen; fibronectin; ECM binding; mucin; antibiotic resistance; lactoferrin
• Status: Published
• ISBN/ISSN/Övrigt: ISSN: 1801-7576