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Feasibility Study of Gel Filtration as a Separation Method for Blood Serum Proteins in Combination with PIXE Analysis

In English

Författare

Summary, in English

The combination between a protein separation technique and the PIXE method has a great potential for large surveys, including thousands of samples, in which multielemental analysis is required. Gel filtration with a Sephadex G-200 gel and a TRIS-acetate buffer was used for separating proteins in human serum. The fractions were doped with an yttrium/vanadium standard and then concentrated and pipeted onto Kimfol™ backing foils. Using the PIXE technique, the distributions of Fe, Cu, Zn, and Se, with respect to molecular size, were found, indicating binding to specific proteins. Sulfur and phosphorus were found to correlate well with the protein content measured by UV-absorption at 280 nm. Further developments and tests on the protein separation technique is required, taking restrictions imposed by the PIXE method into consideration.

Publiceringsår

1987

Språk

Engelska

Sidor

401-409

Publikation/Tidskrift/Serie

Biological Trace Element Research

Volym

12

Issue

1

Dokumenttyp

Artikel i tidskrift

Förlag

Humana Press

Ämne

  • Production Engineering, Human Work Science and Ergonomics
  • Subatomic Physics

Nyckelord

  • metal-binding proteins
  • trace elements
  • protein separation
  • PIXE
  • work environmental health
  • human serum

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1559-0720