Webbläsaren som du använder stöds inte av denna webbplats. Alla versioner av Internet Explorer stöds inte längre, av oss eller Microsoft (läs mer här: * https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Var god och använd en modern webbläsare för att ta del av denna webbplats, som t.ex. nyaste versioner av Edge, Chrome, Firefox eller Safari osv.

Interaction of serum amyloid A with human cystatin Cuidentification of binding sites

In English

Författare

  • Marta Spodzieja
  • Aneta Szymanska
  • Aleksandra Kolodziejczyk
  • Martyna Pradzinska
  • Martyna Maszota
  • Piotr Stefanowicz
  • Zbigniew Szewczuk
  • Anders Grubb
  • Paulina Czaplewska

Summary, in English

Serum amyloid A (SAA) is a multifunctional acute-phase protein whose natural role seems to be participation in many physiologic and pathological processes. Prolonged increased SAA level in a number of chronic inflammatory and neoplastic diseases gives rise to reactive systemic amyloid A amyloidosis, where the N-terminal 76-amino acid residue-long segment of SAA is deposited as amyloid fibrils. Recently, a specific interaction between SAA and the ubiquitous inhibitor of cysteine proteaseshuman cystatin C (hCC)has been described. Here, we report further evidence corroborating this interaction, and the identification of the SAA and hCC binding sites in the SAAhCC complex, using a combination of selective proteolytic excision and high-resolution mass spectrometry. The shortest binding site in the SAA sequence was determined as SAA(86104), whereas the binding site in hCC sequence was identified as hCC(96102). Binding specificities of both interacting sequences were ascertained by affinity experiments (ELISA) and by registration of mass spectrum of SAAhCC complex. Copyright (c) 2012 John Wiley & Sons, Ltd.

Publiceringsår

2012

Språk

Engelska

Sidor

513-524

Publikation/Tidskrift/Serie

Journal of Molecular Recognition

Volym

25

Issue

10

Dokumenttyp

Artikel i tidskrift

Förlag

John Wiley & Sons Inc.

Ämne

  • Pharmacology and Toxicology
  • Medicinal Chemistry

Nyckelord

  • serum amyloid A
  • human cystatin C
  • protein-protein interaction
  • mass
  • spectrometry

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1099-1352