Determination of hexahydrophthalic acid and methylhexahydrophthalic acid in plasma after derivatisation with pentafluorobenzyl bromide using gas chromatography and mass spectrometric detection

Summary, in English

A method for the simultaneous determination of hexahydrophthalic acid (HHP acid) and methylhexahydrophthalic acid (MHHP acid) in human plasma was developed. The procedure was a rapid, single step extractive derivatisation with pentafluorobenzyl bromide as the derivatisation agent. The formed pentafluorobenzyl esters were analysed by gas chromatography-mass spectrometry in negative ion chemical ionisation mode with ammonia as the moderating gas. Deuterium-labeled HHP acid and MHHP acid were used as internal standards. The detection limit was 0.4 ng/ml for HHP acid (m/z 153) and 0.3 ng/ml for MHHP acid (m/z 365). The within-day precision of the method was between 2 and 3% and the between-day precision was between 3 and 12%. The overall recovery was between 65 and 83%. A comparison between HHP acid determinations with a previous and this method showed that the methods gave similar results. The method was applicable for analysis of plasma from occupationally exposed workers.

• Författare: Christian Lindh; Bo A Jönsson
• Avdelning/ar: Avdelningen för arbets- och miljömedicin
• Publiceringsår: 1997
• Språk: Engelska
• Sidor: 331-339
• Publikation/Tidskrift/Serie: Journal of Chromatography. B
• Volym: 691
• Issue: 2
• Länkar: http://dx.doi.org/10.1016/S0378-4347(96)00479-3
• Dokumenttyp: Artikel i tidskrift
• Förlag: Elsevier
• Ämne: Environmental Health and Occupational Health
• Nyckelord: Hexahydrophthalic acid; Methylhexahydrophthalic acid
• Status: Published
• ISBN/ISSN/Övrigt: ISSN: 1387-2273