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Kinetics of Ligand Binding to Membrane Receptors from Equilibrium Fluctuation Analysis of Single Binding Events

  • Anders Gunnarsson
  • Linda Dexlin Mellby
  • Patric Wallin
  • Sofia Svedhem
  • Peter Jonsson
  • Christer Wingren
  • Fredrik Hook
Publiceringsår: 2011
Språk: Engelska
Sidor: 14852-14855
Publikation/Tidskrift/Serie: Journal of the American Chemical Society
Volym: 133
Nummer: 38
Dokumenttyp: Artikel i tidskrift
Förlag: The American Chemical Society


Equilibrium fluctuation analysis of single binding events has been used to extract binding kinetics of ligand interactions with cell-membrane bound receptors. Time-dependent total internal reflection fluorescence (TIRF) imaging was used to extract residence-time statistics of fluorescently stained liposomes derived directly from cell membranes upon their binding to surface-immobilized antibody fragments. The dissociation rate constants for two pharmaceutical relevant antibodies directed against different B-cell expressed membrane proteins was clearly discriminated, and the affinity of the interaction could be determined by inhibiting the interaction with increasing concentrations of soluble antibodies. The single-molecule sensitivity made the analysis possible without overexpressed membrane proteins, which makes the assay attractive in early drug-screening applications.


  • Immunology in the medical area


  • CREATE Health
  • ISSN: 1520-5126

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