Javascript verkar inte påslaget? - Vissa delar av Lunds universitets webbplats fungerar inte optimalt utan javascript, kontrollera din webbläsares inställningar.
Du är här

Identification of prostate specific antigen (PSA) isoforms in complex biological samples utilizing complementary platforms

Publiceringsår: 2010
Språk: Engelska
Sidor: 1137-1147
Publikation/Tidskrift/Serie: Journal of Proteomics2008-01-01+01:00
Volym: 73
Nummer: 6
Dokumenttyp: Artikel i tidskrift
Förlag: Elsevier


Measurements of the prostate-specific antigen (PSA) levels in blood are widely used as diagnostic, predictive and prognostic marker of prostate disease. The selective detection of molecular forms of PSA can contribute clinically to meaningful enhancements of the conventional PSA-test. As it is plausible that an in-depth search for structural variants of PSA gene products may increase our ability to discriminate distinct patho-biological basis and stages of prostate diseases, we have developed a multi-step protocol comprising gel-based methods followed by mass spectrometric identification.

Our current aim was to provide a comprehensive identification of PSA variants occurring in seminal fluid. We provide a proof-of-principle for this multiple step analytical approach to identify multiple PSA variants from complex biological samples that revealed distinct molecular characteristics. In addition, sequence-annotated protein bands in SDS–PAGE gels were compared to those detected by Western blots, and by monitoring the enzymatic activity in zymogram gels, using gelatin as a substrate. The high accuracy annotations were obtained by fast turnaround MALDI-Orbitrap analysis from excised and digested gel bands. Multiple PSA forms were identified utilizing a combination of MASCOT and SEQUEST search engines.


  • Cancer and Oncology
  • Medicinal Chemistry
  • Seminal plasma
  • Mass spectrometry
  • Prostate-specific antigen isoforms
  • MALDI LTQ Orbitrap XL


  • Clinical Chemistry, Malmö
  • ISSN: 1874-3919

Box 117, 221 00 LUND
Telefon 046-222 00 00 (växel)
Telefax 046-222 47 20
lu [at] lu [dot] se

Fakturaadress: Box 188, 221 00 LUND
Organisationsnummer: 202100-3211
Om webbplatsen