Non-invasive, label-free cell counting and quantitative analysis of adherent cells using digital holography
Författare
Summary, in English
Manual cell counting is time consuming and requires a high
degree of skill on behalf of the person performing the count.
Here we use a technique that utilizes digital holography,
allowing label-free and completely non-invasive cell counting
directly in cell culture vessels with adherent viable cells.
The images produced can provide both quantitative and
qualitative phase information from a single hologram.
The recently constructed microscope HolomonitorTM (Phase
Holographic Imaging AB, Lund, Sweden) combines the
commonly used phase contrast microscope with digital
holography, the latter giving us the possibility of achieving
quantitative information on cellular shape, area, confluence
and optical thickness. This project aimed at determining the
accuracy and repeatability of cell counting measurements
using digital holography compared to the conventional
manual cell counting method using a haemocytometer. The
collected datawere also used to determine cell size and cellular
optical thickness.Theresults showthat digital holography can
be used for non-invasive automatic cell counting as precisely
as conventional manual cell counting
degree of skill on behalf of the person performing the count.
Here we use a technique that utilizes digital holography,
allowing label-free and completely non-invasive cell counting
directly in cell culture vessels with adherent viable cells.
The images produced can provide both quantitative and
qualitative phase information from a single hologram.
The recently constructed microscope HolomonitorTM (Phase
Holographic Imaging AB, Lund, Sweden) combines the
commonly used phase contrast microscope with digital
holography, the latter giving us the possibility of achieving
quantitative information on cellular shape, area, confluence
and optical thickness. This project aimed at determining the
accuracy and repeatability of cell counting measurements
using digital holography compared to the conventional
manual cell counting method using a haemocytometer. The
collected datawere also used to determine cell size and cellular
optical thickness.Theresults showthat digital holography can
be used for non-invasive automatic cell counting as precisely
as conventional manual cell counting
Publiceringsår
2008
Språk
Engelska
Sidor
240-247
Publikation/Tidskrift/Serie
Journal of Microscopy
Volym
232
Issue
2
Länkar
Dokumenttyp
Artikel i tidskrift
Förlag
John Wiley & Sons Inc.
Ämne
- Biological Sciences
- Electrical Engineering, Electronic Engineering, Information Engineering
Nyckelord
- Cell analysis
- cellular optical thickness
- cell size
- cell counting
- digital holography
Status
Published
Forskningsgrupp
- Electromagnetic theory
ISBN/ISSN/Övrigt
- ISSN: 0022-2720