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Bacterial Deoxyribonucleoside Kinases Are Poor Suicide Genes in Mammalian Cells

In English

Författare

  • Claire Hebrard
  • Emeline Cros-Perrial
  • Anders Ranegaard Clausen
  • Charles Dumontet
  • Jure Piskur
  • Lars Petter Jordheim

Summary, in English

Transfer of deoxyribonucleoside kinases (dNKs) into cancer cells increases the activity of cytotoxic nucleoside analogues. It has been shown that bacterial dNKs, when introduced into Escherichia coli, sensitize this bacterium toward nucleoside analogues. We studied the possibility of using bacterial dNKs, for example deoxyadenosine kinases (dAKs), to sensitize human cancer cells to gemcitabine. Stable and transient transfections of bacterial dNKs into human cells showed that these were much less active than human and fruitfly dNKs. The fusion of dAK from Bacillus cereus to the green fluorescent protein induced a modest sensitization. Apparently, bacterial dNKs did not get properly expressed or are unstable in the mammalian cell.

Publiceringsår

2009

Språk

Engelska

Sidor

1068-1075

Publikation/Tidskrift/Serie

Nucleosides, Nucleotides & Nucleic Acids

Volym

28

Issue

11-12

Dokumenttyp

Artikel i tidskrift

Förlag

Taylor & Francis

Ämne

  • Biological Sciences

Nyckelord

  • bacteria
  • suicide gene
  • gene therapy
  • Deoxyribonucleoside kinases
  • gemcitabine
  • cancer
  • resistance

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1525-7770