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Versatile microchip utilising ultrasonic standing waves

Publiceringsår: 2005
Språk: Engelska
Sidor: 123-124
Publikation/Tidskrift/Serie: IFMBE Proceedings 2005
Dokumenttyp: Konferensbidrag


This paper presents the concept and initial work on a

microfluidic platform for bead-based analysis of

biological sample. The core technology in this project

is ultrasonic manipulation and trapping of particle in

array configurations by means of acoustic forces. The

platform is ultimately aimed for parallel multistep

bioassays performed on biochemically activated

microbeads (or particles) using submicrolitre sample

volumes. A first prototype with three individually

controlled particle trapping sites has been developed

and evaluated. Standing ultrasonic waves were

generated across a microfluidic channel by integrated

PZT ultrasonic microtransducers. Particles in a fluid

passing a transducer were drawn to pressure minima

in the acoustic field, thereby being trapped and

confined laterally over the transducer. It is

anticipated that acoustic trapping using integrated

transducers can be exploited in miniaturised total

chemical analysis systems (µTAS), where e.g.

microbeads with immobilised antibodies can be

trapped in arrays and subjected to minute amounts of

sample followed by a reaction, detected using

fluorescence. Preliminary results indicate that the

platform is capable of handling live cells as well as

microbeads. A first model bioassay with detection of

fluorescein marked avidin binding to trapped biotin

beads has been evaluated.


  • Medical Engineering


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