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Development of reagents for differential protein quantitation by subtractive parent (precursor) ion scanning

Publiceringsår: 2007
Språk: Engelska
Sidor: 1101-1113
Publikation/Tidskrift/Serie: Journal of Proteome Research
Volym: 6
Nummer: 3
Dokumenttyp: Artikel i tidskrift
Förlag: The American Chemical Society


We present a generic approach for quantitative differential proteomics that reduces data complexity in proteome analysis by automated selection of peptides for MS/MS analysis according to their isotope-labeling ratio. Isotopic reagents were developed that give products which fragment easily to generate a unique pair of signature ions. Using the ion-pair ratio, we show that it is possible to select only BSA peptides (with a 3:1 light heavy isotope ratio) for MS/MS when spiked in a whole yeast extract using Parent (precursor) Ion Quantitation Scanning (PIQS) for MS/MS.


  • Immunology in the medical area
  • precursor-ion
  • reagents
  • mass spectrometry
  • isotope labeling
  • HPLC
  • parent-ion


  • ISSN: 1535-3893

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