Complementary Signaling through flt3 and Interleukin-7 Receptor {alpha} Is Indispensable for Fetal and Adult B Cell Genesis.
Författare
Summary, in English
Extensive studies of mice deficient in one or several cytokine receptors have failed to support an indispensable role of cytokines in development of multiple blood cell lineages. Whereas B1 B cells and Igs are sustained at normal levels throughout life of mice deficient in IL-7, IL-7R{alpha}, common cytokine receptor gamma chain, or flt3 ligand (FL), we report here that adult mice double deficient in IL-7R{alpha} and FL completely lack visible LNs, conventional IgM+ B cells, IgA+ plasma cells, and B1 cells, and consequently produce no Igs. All stages of committed B cell progenitors are undetectable in FL-/- x IL-7R{alpha}-/- BM that also lacks expression of the B cell commitment factor Pax5 and its direct target genes. Furthermore, in contrast to IL-7R{alpha}-/- mice, FL-/- x IL-7R{alpha}-/- mice also lack mature B cells and detectable committed B cell progenitors during fetal development. Thus, signaling through the cytokine tyrosine kinase receptor flt3 and IL-7R{alpha} are indispensable for fetal and adult B cell development.
Avdelning/ar
- Stamcellscentrum (SCC)
- Immunology
- Neurologi, Lund
Publiceringsår
2003
Språk
Engelska
Sidor
1495-1506
Publikation/Tidskrift/Serie
Journal of Experimental Medicine
Volym
198
Issue
10
Fulltext
- Available as PDF - 276 kB
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Dokumenttyp
Artikel i tidskrift
Förlag
Rockefeller University Press
Ämne
- Immunology in the medical area
- Cell and Molecular Biology
- Endocrinology and Diabetes
Nyckelord
- IL-7 receptor
- Flt3 ligand
- B1 cells
- Pax5
- lymphopoiesis
Status
Published
Forskningsgrupp
- Immunology
ISBN/ISSN/Övrigt
- ISSN: 1540-9538