Molecular Recognition Materials Synthesized Based on Small molecules, DNA, and Imprinted Polymers for Bioanalytical Applications
Summary, in English
Boronic acid (BA), which is a small synthetic molecule, was conjugated in nanopores of dendritic fibrous nanosilica (DFNS) through a high-efficiency click reaction and a temperature-responsive polymer intermediate. The developed boronate affinity materials provide more affinity sites for cis-diol enrichment, and the well-defined narrow pores of DFNS provide highly selective affinity binding toward low-molecular-weight cis-diols. Moreover, a BA derivative, 4-vinylphenylboronic acid (VPBA), was employed to develop a fluorescence technique for monitoring molecular imprinting in real time and gaining insights into molecular recognition mechanisms. Molecularly imprinted polymers (MIPs) were synthesized using Alizarin Red S (ARS) as the template and VPBA as the functional monomer. The fluorogenic VPBA–ARS complex enables molecular imprinting signaling. The resulting MIPs also exhibited specific binding toward ARS and served as a fluorescent probe for detecting Cu2+ ions without any tedious sample preparation.
Fluorescein-labeled single-stranded nucleic acid was directly adsorbed on polydopamine-functionalized DFNS to develop a “turn-on” fluorescence biosensor, which functions using an adsorption–quenching–recovery mechanism that enables the simple and highly efficient detection of nucleic acid biomarkers. A proof of concept was demonstrated using microRNA as a biomarker model.
Nucleic acid amplification and MIPs were employed to achieve the low-cost, simple, and reliable detection and quantification of protein biomarkers. MIPs were prepared using Pickering emulsion polymerization and DFNS as a stabilizer to produce surface-accessible binding sites for protein biomarkers. The MIP-induced protein recognition was amplified by the hybridization chain reaction and translated into an easily detectable fluorescence signal.
- Materials Engineering
- Analytical Chemistry
- detection, molecularly imprinted polymers, boronic acid, DNA, dendritic fibrous nanosilica
- ISBN: 978-91-8096-002-1
- ISBN: 978-91-8096-003-8
12 december 2023
Lecture Hall KC:B, Kemicentrum, Naturvetarvägen 14, Faculty of Engineering LTH, Lund University, Lund
- Peter Cormack (Prof.)