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Metabolomic evaluation of pulsed electric field-induced stress on potato tissue

In English

Författare

Summary, in English

Metabolite profiling was used to characterize stress responses of potato tissue subjected to reversible electroporation, providing insights on how potato tissue responds to a physical stimulus such as pulsed electric fields (PEF), which is an artificial stress. Wounded potato tissue was subjected to field strengths ranging from 200 to 400 V/cm, with a single rectangular pulse of 1 ms. Electroporation was demonstrated by propidium iodide staining of the cell nucleae. Metabolic profiling of data obtained through GC/TOF-MS and UPLC/TOF-MS complemented with orthogonal projections to latent structures clustering analysis showed that 24 h after the application of PEF, potato metabolism shows PEF-specific responses characterized by the changes in the hexose pool that may involve starch and ascorbic acid degradation.

Avdelning/ar

Publiceringsår

2009

Språk

Engelska

Sidor

469-479

Publikation/Tidskrift/Serie

Planta

Volym

230

Issue

3

Dokumenttyp

Artikel i tidskrift

Förlag

Springer

Ämne

  • Food Engineering
  • Biological Sciences

Nyckelord

  • Electroporation
  • Cell membrane recovery
  • Metabolic analysis
  • multivariate data analysis
  • OPLS
  • Wounding
  • Stress response

Status

Published

Forskningsgrupp

  • Plant Biology

ISBN/ISSN/Övrigt

  • ISSN: 0032-0935