Respiration of [C-14] alanine by the ectomycorrhizal fungus Paxillus involutus

Summary, in English

The ectomycorrhizal fungus Paxillus involutus efficiently took up exogenously supplied [C-14]alanine and rapidly converted it to pyruvate, citrate, succinate, fumarate and to CO2, thus providing direct evidence for the utilisation of alanine as a respiratory substrate. [C-14]alanine was further actively metabolised to glutamate, glutamine and aspartate. Exposure to aminooxyacetate completely suppressed (CO2)-C-14 evolution and greatly reduced the flow of carbon from [C-14]alanine to tricarboxylic acid cycle intermediates and amino acids, suggesting that alanine aminotransferase plays a pivotal role in alanine metabolism in Paxillus involutus.

• Författare: Michel Chalot; Annick Brun; Roger D. Finlay; Bengt Söderström
• Avdelning/ar: MEMEG; Mikrobiologisk ekologi
• Publiceringsår: 1994
• Språk: Engelska
• Sidor: 87-91
• Publikation/Tidskrift/Serie: FEMS Microbiology Letters
• Volym: 121
• Issue: 1
• Länkar: http://dx.doi.org/10.1111/j.1574-6968.1994.tb07080.x
• Dokumenttyp: Artikel i tidskrift
• Förlag: Oxford University Press
• Ämne: Biological Sciences
• Nyckelord: Amino acid respiration; Aminooxyacetate; [14C]alanine; Ectomycorrhizal fungus; Paxillus involutus; TCA cycle
• Status: Published
• Forskningsgrupp: Microbial Ecology
• ISBN/ISSN/Övrigt: ISSN: 1574-6968