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Influence of bioreactor scale and complex medium on probing control of glucose feeding in cultivations of recombinant strains of Escherichia coli

  • Stephane Velut
  • Andreas Castan
  • Kevin A. Short
  • Jan Peter Axelsson
  • Per Hagander
  • Barry A. Zditosky
  • Christopher W. Rysenga
  • Lena de Maré
  • Jan Haglund
Publiceringsår: 2007
Språk: Engelska
Sidor: 816-824
Publikation/Tidskrift/Serie: Biotechnology and Bioengineering
Volym: 97
Nummer: 4
Dokumenttyp: Artikel i tidskrift
Förlag: John Wiley & Sons


The objective of this work was to evaluate the performance of a feedback glucose control strategy (the probing strategy) in production relevant bioreactors with complex and mineral media. Experimental results from fedbatch cultivations with two recombinant Escherichia coli constructs expressing two different human therapeutic proteins were used to assess the performance and limitations of the glucose probing technique. Even though the performance of the probing strategy was affected by scale and complex media, this methodology rapidly identified a glucose feed protocol similar to an experimentally derived feed regime. This methodology may serve as a powerful tool for industrial process development and in optimization of glucose feed regimes when transferring process technology from one bioreactor system to another.


  • Control Engineering
  • probing control
  • feeding strategy
  • fed-batch
  • fermentations
  • industrial
  • bioreactor
  • complex medium


  • ISSN: 1097-0290

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