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Macro-/nanoporous silicon as a support for high-performance protein microarrays

Författare

Summary, in English

The present work demonstrates the possibilities of using macroporous silicon as a substrate for highly sensitive protein chip applications. The formation of 3D porous silicon structures was performed by electrochemical dissolution of monocrystalline silicon. The fabricated macroporous silicon network has a rigid spongelike structure showing high uniformity and mechanical stability. The microfluidic properties of the substrates were found to be essential for a good bioassay performance. Small spot area, good spot reproducibility, and homogeneous spot profiles were demonstrated on the substrates for immobilized aRIgG. Water contact angles were measured on the porous surface and compared to that of planar silicon, silanized glass, and ordinary microscope glass slides. The effect of the porous surface on the performance of a model IgG-binding immunoassay is presented. aRIgG was microdispensed onto the chip surface forming a microarray of spots with high affinity for the target analyte. The dispensing was performed using an in-house-developed piezoelectric flow-through dispenser. Each spot was formed by a single droplet (100 pL) at each position. The macroporous silicon allowed a high-density microarraying with spot densities up to 4400 spots/cm(2) in human plasma samples without cross-talk and consumption of only 0.6 pmol of antibodies/1-cm(2) array. Antigen levels down to 70 pM were detected.

Publiceringsår

2003

Språk

Engelska

Sidor

6968-6974

Publikation/Tidskrift/Serie

Analytical Chemistry

Volym

75

Issue

24

Dokumenttyp

Artikel i tidskrift

Förlag

The American Chemical Society (ACS)

Ämne

  • Analytical Chemistry

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1520-6882