Silicon microstructures for high-speed and high-sensitivity protein identifications

Summary, in English

Silicon microtechnology has been used to develop a microstructure toolbox in order to enable high accuracy protein identification. During the last 2 years we developed and applied monocrystalline silicon structures and established new automated protein analysis platforms, The development of a high throughput protein platform is presented where fully automated protein identifications are performed. It includes the reduction and alkylation of the protein sample in a standard 96- or 384-well plate format prior to injection of 1 mul samples into the continuous flow based microtechnology platform. The processed sample is transferred to a microchip nanovial array target using piezoelectric microdispensing. identification is made by MALDI-TOF MS and a database search. After the initial sample reduction and alkylation period of 50 min the platform can digest and process protein samples at a speed of 100 samples in 210 min. An optional configuration of the platform, operating the dispenser in the 'static mode', enables on-target enrichment of low abundant proteins and peptides e.g. from 2DE samples. This makes detection at the low attomole level possible. (C) 2001 Elsevier Science B.V. All rights reserved.

• Författare: Thomas Laurell; J. Nilsson; György Marko-Varga
• Avdelning/ar: Avdelningen för Biomedicinsk teknik
• Publiceringsår: 2001
• Språk: Engelska
• Sidor: 217-232
• Publikation/Tidskrift/Serie: Journal of Chromatography. B, Biomedical Sciences and Applications
• Volym: 752
• Issue: 2
• Länkar: http://dx.doi.org/10.1016/s0378-4347(00)00358-3
• Dokumenttyp: Artikel i tidskrift
• Förlag: Elsevier
• Ämne: Medical Engineering
• Nyckelord: silicon microstructures; proteins; FLIGHT MASS-SPECTROMETRY; FLOW-THROUGH DISPENSER; SAMPLE PREPARATION; POROUS SILICON; MATRIX; MALDI
• Status: Published
• ISBN/ISSN/Övrigt: ISSN: 1387-2273