Webbläsaren som du använder stöds inte av denna webbplats. Alla versioner av Internet Explorer stöds inte längre, av oss eller Microsoft (läs mer här: * https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Var god och använd en modern webbläsare för att ta del av denna webbplats, som t.ex. nyaste versioner av Edge, Chrome, Firefox eller Safari osv.

Integrated protein microchip assay with dual fluorescent- and MALDI read-out

In English

Författare

Summary, in English

A pore chip protein array (PCPA) concept based on a dual readout configuration, fluorescence imaging, and MALDI-TOF MS has been developed. Highly packed, (>4000 Spots/cm(2)), antibody arrays were dispensed on the porous chip by using a piezo-electric microdispenser. Sandwich assay was made after blocking by addition of a secondary antibody either lgG-FITC-labeled or anti-Ang II. The antigen in the first system was a large protein (lgG), and in the other system, a FITC marked peptide Angiotensin II (Ang II) was used. Ang II antibodies showed specificity for Ang II, while the Ang I antibodies showed binding properties for Ang I, II, and Renin. Fluorescence and MALDI TOF MS read-out was made for lgG and Ang II. A major advantage of the dual read-out PCPA approach is that both affinity binding and mass identity are derived. Detection limits for Ang II on the chip is as low as 500 zmol (Ang II).

Publiceringsår

2004

Språk

Engelska

Sidor

988-994

Publikation/Tidskrift/Serie

Journal of Proteome Research

Volym

3

Issue

5

Dokumenttyp

Artikel i tidskrift

Förlag

The American Chemical Society (ACS)

Ämne

  • Analytical Chemistry
  • Medical Engineering

Nyckelord

  • matrix-assisted laser desorption ionization time-of-flight

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1535-3893