Quantification of Membrane Proteins Using Nonspecific Protease Digestions

Summary, in English

We present a mass spectrometry-based method for the identification and quantification of membrane proteins using the low-specificity protease Proteinase K, at very high pH, to digest proteins isolated by a modified SDS-PAGE protocol. The resulting peptides are modified with a fragmentation-directing isotope labeled tag. We apply the method to quantify differences in membrane protein expression of Bacillus subtilis grown in the presence or absence of glucose.

• Författare: Maria Bendz; Mirja Carlsson Möller; Giorgio Arrigoni; Åsa Wåhlander; Roberto Stella; Salvatore Cappadona; Fredrik Levander; Lars Hederstedt; Peter James
• Avdelning/ar: Molekylär cellbiologi; Institutionen för immunteknologi
• Publiceringsår: 2009
• Språk: Engelska
• Sidor: 5666-5673
• Publikation/Tidskrift/Serie: Journal of Proteome Research
• Volym: 8
• Issue: 12
• Länkar: http://dx.doi.org/10.1021/pr900741t
• Dokumenttyp: Artikel i tidskrift
• Förlag: The American Chemical Society (ACS)
• Ämne: Immunology in the medical area
• Nyckelord: membrane proteins; N-terminal labeling; quantification; relative; proteinase K; Bacillus subtilis
• Status: Published
• ISBN/ISSN/Övrigt: ISSN: 1535-3893