Calpain inhibitors delay injury-induced apoptosis in adult mouse spinal cord motor neurons
Författare
Summary, in English
Here, we investigated the effect of calpain inhibitors on apoptosis in organotypic adult spinal cord slices from mice. An increase in calpain I immunoreactivity was found in the nuclei of motor neurons from slices cultured for 30 min. After 4 h, the immunopositive motor neurons exhibited apoptotic changes including nuclear and chromatin condensation. Eight hours after excision, most motor neurons showed nuclear apoptotic features. Two calpain inhibitors, leupeptin and calpain inhibitor XI, inhibited apoptosis in the motor neurons while the caspase inhibitor Z-VAD.fmk had no effect. Leupeptin, but not calpain inhibitor XI and Z-VAD.fmk, also inhibited nucleosomal DNA fragmentation. These results suggest the involvement of calpain I in the induction of apoptosis in motor neurons of adult spinal cord and that apoptosis can be triggered independent of caspase activation.
Avdelning/ar
Publiceringsår
2006
Språk
Engelska
Sidor
761-765
Publikation/Tidskrift/Serie
NeuroReport
Volym
17
Issue
8
Länkar
Dokumenttyp
Artikel i tidskrift
Förlag
Lippincott Williams & Wilkins
Ämne
- Neurosciences
Nyckelord
- spinal cord
- culture
- organ
- motor neurons
- caspase
- calpain inhibitor
- apoptosis
- calpain
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 1473-558X