Webbläsaren som du använder stöds inte av denna webbplats. Alla versioner av Internet Explorer stöds inte längre, av oss eller Microsoft (läs mer här: * https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Var god och använd en modern webbläsare för att ta del av denna webbplats, som t.ex. nyaste versioner av Edge, Chrome, Firefox eller Safari osv.

The search for a peptide ligand targeting the lipolytic enzyme cutinase

In English

Författare

Summary, in English

A constrained nonapeptide phage display library was evaluated as a potential source of affinity ligand(s) for purification of cutinase, a lipolytic enzyme. After seven cycles of biopanning, 500 clones were isolated and individually tested for their capability to interact with cutinase. Three out of six sequenced clones carrying a cutinase-specific constrained peptide showed the same insert sequence (CRLHHWRYC). Sequences of two of the other clones highlighted a LXXW motif as a critical determinant in the make-up of a cutinase-specific sequence. Although the affinity of the most commonly found peptide for cutinase is low, we suggest that LXXW motif may be a suitable starting point in the development of affinity peptides suitable for use in the study and purification of cutinase.

Publiceringsår

2003

Språk

Engelska

Sidor

244-249

Publikation/Tidskrift/Serie

Enzyme and Microbial Technology

Volym

33

Issue

2-3

Dokumenttyp

Artikel i tidskrift

Förlag

Elsevier

Ämne

  • Immunology in the medical area
  • Industrial Biotechnology

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 0141-0229