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Interaction between phosphofructokinase and aldolase from Saccharomyces cevisiae studied by aqueous two-phase partitioning

Publiceringsår: 2001
Språk: Engelska
Sidor: 341-348
Publikation/Tidskrift/Serie: Journal of Chromatography. B, Biomedical Sciences and Applications
Volym: 751
Nummer: 2
Dokumenttyp: Artikel i tidskrift
Förlag: Elsevier


Phosphofructokinase (EC and aldolase (EC have been highly purified from Saccharomyces cerevisiae

by improved protocols. Partitioning of the enzymes in aqueous polymer two-phase systems was used to detect complex

formation. The partition of each enzyme was found to be affected by the presence of the other enzyme. AMP affected the

partition of the individual enzymes as well as the mixture of the two. The activities of the respective enzymes were

stimulated in the putative complex in an AMP-dependent manner. Two strictly conserved residues belonging to an acidic

surface loop of class II aldolases, are a potential site for electrostatic interaction with the positively charged regions close to

the active site in phosphofructokinase. Ó 2001 Elsevier Science B.V. All rights reserved.


  • Biological Sciences


  • ISSN: 1387-2273

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