Pasteurella multocida thymidine kinase 1 efficiently activates pyrimidine nucleoside analogs.

Summary, in English

In the Pasteurella multocida genome only one putative deoxyribonucleoside kinase encoding gene, for thymidine kinase 1 (PmTK1), was identified. The PmTK1 gene was sub-cloned into Escherichia coli KY895 and it sensitized the host towards 2',2'-difluoro-deoxycytidine (gemcitabine, dFdC), 3'-azido-thymidine (AZT) and 5-fluoro-deoxyuridine (5F-dU). PmTK1 was over-expressed and purified with two different tags. Apparently, deoxyuridine (dU), and not thymidine (dT), is the preferred substrate. We suggest that PmTK1s could be employed as a species-specific activator of uracil-based nucleoside antibiotics.

• Författare: Anders Ranegaard Clausen; S A L Al Meani; Jure Piskur
• Avdelning/ar: Biologiska institutionen; Molekylär cellbiologi
• Publiceringsår: 2010
• Språk: Engelska
• Sidor: 359-362
• Publikation/Tidskrift/Serie: Nucleosides, Nucleotides & Nucleic Acids
• Volym: 29
• Issue: 4-6
• Länkar: http://dx.doi.org/10.1080/15257771003729716
• Dokumenttyp: Artikel i tidskrift
• Förlag: Taylor & Francis
• Ämne: Biological Sciences
• Status: Published
• ISBN/ISSN/Övrigt: ISSN: 1525-7770