Early B-cell factor (o/e-1) is a promoter of adipogenesis and involved in control of genes important for terminal adipocyte differentiation.

Summary, in English

Olf-1/early B-cell factor (O/E-1) is a transcription factor important for B-lymphocyte and neuronal gene regulation. Here we report that all three known O/E genes (O/E-1, -2, and -3) are expressed in mouse adipose tissue and are upregulated during adipocyte differentiation. Forced expression of O/E-1 in either the preadipocyte cell line 3T3-L1 or mouse embryonic fibroblasts augmented adipogenesis, and constitutive expression of O/E-1 in uncommitted NIH 3T3 fibroblasts led to initiation of adipocyte differentiation. Furthermore, a dominant negative form of O/E-1 partially suppressed 3T3-L1 adipogenesis, indicating that expression from endogenous O/E target genes is required for 3T3-L1 terminal differentiation. Thus, our data point to the importance of O/E target genes for adipocyte differentiation and suggest a novel role for O/E-1 as an initiator and stimulator of adipogenesis.

• Författare: Peter Åkerblad; Ulrika Lind; David Liberg; Krister Bamberg; Mikael Sigvardsson
• Avdelning/ar: Immunology; Stamcellscentrum (SCC)
• Publiceringsår: 2002
• Språk: Engelska
• Sidor: 8015-8025
• Publikation/Tidskrift/Serie: Molecular and Cellular Biology
• Volym: 22
• Issue: 22
• Fulltext: - 658 kB; Download statistics
• Länkar: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12391167&dopt=Abstract; http://mcb.asm.org/cgi/content/full/22/22/8015?view=full&pmid=12391167; http://dx.doi.org/10.1128/MCB.22.22.8015-8025.2002
• Dokumenttyp: Artikel i tidskrift
• Förlag: American Society for Microbiology
• Ämne: Cell and Molecular Biology
• Status: Published
• Forskningsgrupp: Immunology
• ISBN/ISSN/Övrigt: ISSN: 0270-7306