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On-line Detection of Acetate Formation in Escherichia coli Cultures using Dissolved Oxygen Responses to Feed Transients

In English

Författare

Summary, in English

Recombinant protein production in <i>Escherichia coli</i> can be significantly reduced by acetate accumulation. It is demonstrated that acetate production can be detected on-line with a standard dissolved oxygen sensor by superimposing short pulses to the substrate feed rate. Assuming that acetate formation is linked to a respiratory limitation, a model for dissolved oxygen responses to transients in substrate feed rate is derived. The model predicts a clear change in the character of the transient response when acetate formation starts. The predicted effect was verified in fed-batch cultivations of E. coli TOPP1 and E. coli BL21(DE3), both before and after induction of recombinant protein production. It was also observed that the critical specific glucose uptake rate, at which acetate formation starts, was significantly decreased after induction. On-line detection of acetate formation with a standard sensor opens up new possibilities for feedback control of substrate feeding.

Publiceringsår

1999

Språk

Engelska

Sidor

590-598

Publikation/Tidskrift/Serie

Biotechnology and Bioengineering

Volym

64

Issue

5

Dokumenttyp

Artikel i tidskrift

Förlag

John Wiley & Sons Inc.

Ämne

  • Industrial Biotechnology
  • Control Engineering

Nyckelord

  • on-line detection
  • acetate
  • \emph{Escherichia coli}
  • dissolved oxygen
  • transients
  • mathematical model

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1097-0290