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In vitro molecular evolution of antibody genes mimicking receptor revision

In English

Författare

Summary, in English

Antibody evolution in vivo proceeds mainly by stepwise improvements, accomplished by single base pair substitutions. Lately, receptor revision, i.e. exchange of large parts of the V gene for another sequence, has been suggested to provide a complementary route for affinity maturation. By employing a receptor revision like evolution process in vitro using combinatorial libraries and phage display selection, we demonstrate here that maturation of a clone may preferentially proceed through exchange of a large gene segment rather than via minor sequence changes. These modifications of a CD40-specific human antibody fragment outline how receptor revision like events may provide an advantage to a particular clonotype put under selective pressure. (C) 2002 Elsevier Science Ltd. All rights reserved.

Publiceringsår

2002

Språk

Engelska

Sidor

349-356

Publikation/Tidskrift/Serie

Molecular Immunology

Volym

39

Issue

5-6

Dokumenttyp

Artikel i tidskrift

Förlag

Pergamon Press Ltd.

Ämne

  • Immunology in the medical area

Nyckelord

  • phage display
  • affinity maturation
  • antibody
  • receptor revision
  • somatic hypermutation

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1872-9142