Enzyme conformational alterations detected by partition column chromatography
Författare
Summary, in English
In this paper, we demonstrate the ability of liquid-liquid partition chromatography (LLPC) to detect conformational alterations occurring in well-characterized enzymes. The conformational changes induced in dehydrogenases such as alcohol dehydrogenase (ADH), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), lactate dehydrogenases (LDH) and malate dehydrogenase (MDH) upon binding of ligand(s) were detectable by LLPC. The ligand-dependent equilibrium between two forms of citrate synthase (CS), glutamate-oxaloacetate transaminase (GOT), hexokinase (HK) and 3-phosphoglycerate kinase (PGK) could also be demonstrated. Furthermore, different conformational forms of some of the apoenzymes could also be detected and separated by LLPC. The results obtained here are discussed in relation to those obtained by other methods.
Avdelning/ar
Publiceringsår
1996
Språk
Engelska
Sidor
113-122
Publikation/Tidskrift/Serie
Journal of Chromatography. B
Volym
680
Issue
1-2
Dokumenttyp
Artikel i tidskrift
Förlag
Elsevier
Ämne
- Biological Sciences
Nyckelord
- Partition column chromatography
- Conformational changes
- Enzymes
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 1572-6495