Use of chemically extracted muscle grafts to repair extended nerve defects in rats
Författare
Summary, in English
Nerve regeneration, measured as axonal outgrowth, Schwann cell migration, macrophage invasion, and neovascularisation, was compared after repair of a 15 mm gap in rats' sciatic nerves using autologous muscle grafts made acellular either by freezing and thawing or by chemical extraction. Both extracted and freeze-thawed acellular muscle grafts could be used to bridge the defect. However, axons and Schwann cells, as shown by immunohistochemical staining for neurofilaments and S-100 protein, respectively, grew faster into the extracted muscle grafts than into the freeze-thawed acellular muscle grafts and somewhat more axons were observed in the former graft. There were no significant differences between the two graft types with respect to neovascularisation as showed by staining for endothelial alkaline phosphatase, and limited differences concerning invasion of macrophages (ED1 and ED2) as detected by immunocytochemistry. The results showed that chemically extracted muscle grafts could be used to bridge an extended nerve defect and that such grafts in some aspects were superior to freeze-thawed muscle grafts for extended gaps.
Avdelning/ar
Publiceringsår
2001
Språk
Engelska
Sidor
337-345
Publikation/Tidskrift/Serie
Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery
Volym
35
Issue
4
Dokumenttyp
Artikel i tidskrift
Förlag
Taylor & Francis
Ämne
- Surgery
Nyckelord
- Muscle
- Graft
- Acellular
- Nerve
- Regeneration
- Schwann
- Cell
- Macrophages
- Immunocytochemistry
- Axons
- Repair
Status
Published
Forskningsgrupp
- Hand Surgery, Malmö
ISBN/ISSN/Övrigt
- ISSN: 1651-2073