Transition of the ability to generate petites in the Saccharomyces/Kluyveromyces complex

Summary, in English

Petite-positivity - the ability to tolerate the loss of mtDNA - was examined after the treatment with ethidium bromide (EB) in over hundred isolates from the Saccharomyces/Kluyveromyces complex. The identity of petite mutants was confirmed by the loss of specific mtDNA DAPI staining patterns. Besides unequivocal petite-positive and petite-negative phenotypes, a few species exhibited temperature sensitive petite positive phenotype and petiteness of a few other species could be observed only at the elevated EB concentrations. Several yeast species displayed a mixed 'moot' phenotype, where a major part of the population did not tolerate the loss of mtDNA but several cells did. The genera from postwhole-genome duplication lineages (Saccharomyces, Kazachstania, Naumovia, Nakaseomyces) were invariably petite-positive. However, petite-positive traits could also be observed among the prewhole-genome duplication species.

• Författare: Veronika Fekete; Maria Cierna; Silvia Poláková; Jure Piskur; Pavol Sulo
• Avdelning/ar: Biologiska institutionen; Molekylär cellbiologi
• Publiceringsår: 2007
• Språk: Engelska
• Sidor: 1237-1247
• Publikation/Tidskrift/Serie: FEMS Yeast Research
• Volym: 7
• Issue: 8
• Länkar: http://dx.doi.org/10.1111/j.1567-1364.2007.00287.x
• Dokumenttyp: Artikel i tidskrift
• Förlag: Oxford University Press
• Ämne: Biological Sciences
• Nyckelord: ethidium; Saccharomyces/Kluyveromyces complex; petite mutation; mtDNA; bromide; mitochondria
• Status: Published
• ISBN/ISSN/Övrigt: ISSN: 1567-1364