The Na(+) transport in Gram-positive bacteria defect in the Mrp antiporter complex measured with (23)Na-NMR.

Summary, in English

(23)Na-NMR has previously been used to monitor Na(+) translocation across membranes in Gram-negative bacteria and in various other organelles and liposomes using a membrane-impermeable shift reagent to resolve the signals resulting from internal and external Na(+). In this work, the (23)Na-NMR method was adapted for measurements of internal Na(+) concentration in the Gram-positive bacterium Bacillus subtilis, with the aim of assessing the Na(+) translocation activity of the Mrp antiporter complex, a member of the Cation Proton Antiporter-3 (CPA-3) family. The sodium sensitive growth phenotype observed in a B. subtilis strain with the gene encoding MrpA deleted, could indeed be correlated to the inability of this strain to maintain a lower internal than external Na(+) concentration.

• Författare: Kamil Gorecki; Cecilia Hägerhäll; Torbjörn Drakenberg
• Avdelning/ar: Biokemi och Strukturbiologi; Biofysikalisk kemi
• Publiceringsår: 2014
• Språk: Engelska
• Sidor: 80-86
• Publikation/Tidskrift/Serie: Analytical Biochemistry
• Volym: 445
• Issue: Online 15 October 2013
• Länkar: http://dx.doi.org/10.1016/j.ab.2013.10.003
• Dokumenttyp: Artikel i tidskrift
• Förlag: Elsevier
• Ämne: Biological Sciences; Physical Chemistry
• Status: Published
• ISBN/ISSN/Övrigt: ISSN: 1096-0309