Human monoclonal antibodies against an epitope on the class 5c outer membrane protein common to many pathogenic strains of Neisseria meningitidis
Författare
Summary, in English
Neisseria meningitidis is a causative agent of meningitis. Despite vaccination programs, it still causes a large number of deaths in young children. Early diagnosis followed by passive immunization with human monoclonal antibodies could be an approach to effective therapy. Peripheral blood lymphocytes from normal, healthy blood donors and from vaccinated individuals were immunized in vitro, using outer membrane proteins purified from A. meningitidis B:4:P1,15. The immunized human B cells were Epstein-Barr virus transformed and fused to a heteromyeloma. Several stable human hybridoma cell lines were established and two, secreting antibodies against the 31-kDa class 5c outer membrane protein, were characterized further. The human antibodies were of IgG1 and IgG3 isotypes, with κ light chains. The recognized epitope was commonly found among pathogenic strains of N. meningitidis; thus, these human monoclonal antibodies may be important in the evaluation of N. meningitidis infections.
Avdelning/ar
Publiceringsår
1992
Språk
Engelska
Sidor
1322-1328
Publikation/Tidskrift/Serie
Journal of Infectious Diseases
Volym
166
Issue
6
Dokumenttyp
Artikel i tidskrift
Förlag
Oxford University Press
Ämne
- Infectious Medicine
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 0022-1899