New Set-up for Capillary Isoelectric Focusing in Uncoated Capillaries
Författare
Summary, in English
Substituted aminomethylphenol dyes, low-molecular-mass isoelectric point (pI ) markers and hemoglobin samples from normal individuals and diabetic patients were used to test a new set-up of capillary isoelectric focusing (cIEF) in uncoated capillaries. In previous cIEF methods, a mixture of sample components and carrier ampholytes was applied in the capillary and analyzed. In the new set-up a fractionated injection protocol is used to apply a ‘sandwich’ ampholyte–sample–ampholyte plug in the capillary for analysis. This new set-up allows the separation of amphoteric compounds having pI values outside the pH region of the ampholytes applied in the capillary with high precision. The high resolution power of this technique was proven with the analysis of hemoglobin variants.
Publiceringsår
1998
Språk
Engelska
Sidor
349-360
Publikation/Tidskrift/Serie
Journal of Chromatography A
Volym
813
Issue
2
Dokumenttyp
Artikel i tidskrift
Förlag
Elsevier
Ämne
- Medical Engineering
Nyckelord
- Isoelectric focusing
- Sandwich injection
- Injection methods
- Haemoglobin
- Dyes
- Nitrophenols
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 0021-9673