Rhodothermus marinus: a thermophilic bacterium producing dimeric and hexameric citrate synthase isoenzymes.
Författare
Summary, in English
Two separate citrate synthases from the extremely thermophilic bacterium Rhodothermus marinus have been identified and purified. One of the enzymes is a hexameric protein and is the first thermostable, hexameric citrate synthase to be isolated. The other is a dimeric enzyme, which is also thermostable but possesses both citrate synthase and 2-methyl citrate synthase activities. 2-Methyl citrate synthase uses propionyl-coenzyme A as one of its substrates and in Escherichia coli, for example, it has been implicated in the metabolism of propionate. However, no growth of R. marinus was observed using minimal medium with propionate as the sole carbon source, and both hexameric and dimeric enzymes were produced irrespective of whether propionate was included in the growth medium. The data are discussed with respect to the evolutionary relationships between the known hexameric and dimeric citrate synthases and 2-methyl citrate synthase.
Avdelning/ar
Publiceringsår
2002
Språk
Engelska
Sidor
51-56
Publikation/Tidskrift/Serie
Extremophiles
Volym
6
Issue
1
Dokumenttyp
Artikel i tidskrift
Förlag
Springer
Ämne
- Industrial Biotechnology
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 1433-4909