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Cloning and characterization of a promoter flanking the early B cell factor (EBF) gene indicates roles for E-proteins and autoregulation in the control of EBF expression.

Författare:
Publiceringsår: 2002
Språk: Engelska
Sidor: 261-270
Publikation/Tidskrift/Serie: J Immunol
Volym: 169
Nummer: 1
Dokumenttyp: Artikel
Förlag: American Association of Immunologists

Sammanfattning

The early B cell factor (EBF) is a transcription factor shown crucial for the development of B lymphocytes. The protein is expressed from the earliest stages of B cell development until the mature B cell stage, but the control elements responsible for the regulation of the gene are unknown. In this study, we report of the identification of a promoter region flanking the EBF gene. Several transcription start sites were identified by primer extension analysis in a region approximately 3.1 kb from the predicted ATG. Transient transfections revealed that this region was able to stimulate transcription of a reporter gene in B lymphoid and to a lesser extent, myeloid cells, but not in a pre-T cell line. The promoter was also able to functionally interact with E47, suggesting that the EBF gene may be a direct target for activation by E-proteins. In addition, functional binding of EBF to its own promoter was confirmed by EMSA and transfection assays indicating that the EBF protein may be involved in an autoregulatory loop. Finally, a tissue-restricted factor was able to bind an upstream regulatory region in B-lineage cells, further supporting the idea that the cloned promoter participates in the regulation of stage and lineage specific expression of the EBF gene.

Disputation

Nyckelord

  • Biology and Life Sciences
  • Medicine and Health Sciences
  • Transcription Factors : physiology
  • Trans-Activators : biosynthesis
  • Inbred BALB C
  • Mice
  • Cells
  • Nuclear Proteins : metabolism
  • Organ Specificity : genetics
  • Protein Binding : immunology
  • Organ Specificity : immunology
  • Trans-Activators : genetics
  • E-Box Elements : genetics
  • E-Box Elements : immunology
  • Exons : genetics
  • Exons : immunology
  • Hela Cells
  • Helix-Loop-Helix Motifs : genetics
  • Helix-Loop-Helix Motifs : immunology
  • Human
  • Molecular Sequence Data
  • Nuclear Proteins : physiology
  • Promoter Regions (Genetics) : immunology
  • Protein Binding : genetics
  • Support
  • Non-U.S. Gov't
  • Trans-Activators : chemistry
  • Trans-Activators : physiology
  • Nuclear Proteins : genetics
  • Inbred C57BL
  • DNA-Binding Proteins : physiology
  • DNA-Binding Proteins : genetics
  • DNA-Binding Proteins : chemistry
  • DNA-Binding Proteins : biosynthesis
  • Molecular
  • Cloning
  • Cultured
  • 5' Untranslated Regions : genetics
  • 5' Untranslated Regions : immunology
  • Amino Acid Sequence
  • Animal
  • B-Lymphocytes : immunology
  • B-Lymphocytes : metabolism
  • Base Composition
  • Base Sequence

Övrigt

Published
Yes
  • ISSN: 0022-1767

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