Cloning and characterization of a promoter flanking the early B cell factor (EBF) gene indicates roles for E-proteins and autoregulation in the control of EBF expression.
Författare
Summary, in English
The early B cell factor (EBF) is a transcription factor shown crucial for the development of B lymphocytes. The protein is expressed from the earliest stages of B cell development until the mature B cell stage, but the control elements responsible for the regulation of the gene are unknown. In this study, we report of the identification of a promoter region flanking the EBF gene. Several transcription start sites were identified by primer extension analysis in a region approximately 3.1 kb from the predicted ATG. Transient transfections revealed that this region was able to stimulate transcription of a reporter gene in B lymphoid and to a lesser extent, myeloid cells, but not in a pre-T cell line. The promoter was also able to functionally interact with E47, suggesting that the EBF gene may be a direct target for activation by E-proteins. In addition, functional binding of EBF to its own promoter was confirmed by EMSA and transfection assays indicating that the EBF protein may be involved in an autoregulatory loop. Finally, a tissue-restricted factor was able to bind an upstream regulatory region in B-lineage cells, further supporting the idea that the cloned promoter participates in the regulation of stage and lineage specific expression of the EBF gene.
Publiceringsår
2002
Språk
Engelska
Sidor
261-270
Publikation/Tidskrift/Serie
Journal of Immunology
Volym
169
Issue
1
Länkar
Dokumenttyp
Artikel i tidskrift
Förlag
American Association of Immunologists
Ämne
- Immunology in the medical area
Nyckelord
- Transcription Factors : physiology
- Trans-Activators : biosynthesis
- Inbred BALB C
- Mice
- Cells
- Nuclear Proteins : metabolism
- Organ Specificity : genetics
- Protein Binding : immunology
- Organ Specificity : immunology
- Trans-Activators : genetics
- E-Box Elements : genetics
- E-Box Elements : immunology
- Exons : genetics
- Exons : immunology
- Hela Cells
- Helix-Loop-Helix Motifs : genetics
- Helix-Loop-Helix Motifs : immunology
- Human
- Molecular Sequence Data
- Nuclear Proteins : physiology
- Promoter Regions (Genetics) : immunology
- Protein Binding : genetics
- Support
- Non-U.S. Gov't
- Trans-Activators : chemistry
- Trans-Activators : physiology
- Nuclear Proteins : genetics
- Inbred C57BL
- DNA-Binding Proteins : physiology
- DNA-Binding Proteins : genetics
- DNA-Binding Proteins : chemistry
- DNA-Binding Proteins : biosynthesis
- Molecular
- Cloning
- Cultured
- 5' Untranslated Regions : genetics
- 5' Untranslated Regions : immunology
- Amino Acid Sequence
- Animal
- B-Lymphocytes : immunology
- B-Lymphocytes : metabolism
- Base Composition
- Base Sequence
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 1550-6606