Dispersion of viable pig liver cells with collagenase

Summary, in English

Viable suspended hepatocytes were prepared from surgical biopsy specimens of pig and human liver by digestion with collagenase. Initial perfusion of the tissue through cannulated blood vessels with 0.5 mM EGTA followed by 0.2% collagenase gave the best results. 20−870 × 106 cells of which 60–95 % excluded trypan blue were obtained from 5–30 g pig liver pieces, while results with human liver specimens were usually less satisfactory. In some experiments, however, viable cells, as judged by vital stain exclusion and ability to synthesize lipids were obtained in sufficient yield. In the pig hepatocytes glycerolipid synthesis from [3H] glycerol and oxidation and esterification of [14C] oleic acid had the same characteristics as those observed earlier in rat hepatocytes.

• Författare: Per Belfrage; Bengt Börjesson; Inga Hägerstrand; Åke Nilsson; Arne Olsson; Thomas Wiebe; Björn Akesson
• Avdelning/ar: Pediatrik, Lund; Medicin/akutsjukvård, Lund; Kirurgi, Lund
• Publiceringsår: 1975
• Språk: Engelska
• Sidor: 1219-1225
• Publikation/Tidskrift/Serie: Life Sciences
• Volym: 17
• Issue: 8
• Länkar: http://dx.doi.org/10.1016/0024-3205(75)90130-7
• Dokumenttyp: Artikel i tidskrift
• Förlag: Elsevier
• Ämne: Other Clinical Medicine; Cancer and Oncology; Surgery; Pediatrics; Cell and Molecular Biology
• Status: Published
• ISBN/ISSN/Övrigt: ISSN: 1879-0631