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Beta 1 integrin-mediated collagen gel contraction is stimulated by PDGF

In English

Författare

  • Donald Gullberg
  • Anders Tingström
  • Ann-Charlotte Thuresson
  • Lennart Olsson
  • Louis Terracio
  • Thomas K Borg
  • Kristofer Rubin

Summary, in English

The attachment of primary rat hepatocytes and fibroblasts to collagen type I is mediated by non-RGD-dependent beta 1 integrin matrix receptors. In this report we describe a novel 96-well microtiter plate assay for the quantification of fibroblast-mediated contraction of floating collagen type I gels. Fetal calf serum and platelet-derived growth factor (PDGF), but not transforming growth factor-beta 1, stimulated primary rat heart fibroblasts and normal human diploid fibroblasts (AG 1518) to contract collagen gels to less than 10% of the initial gel volume within a 24-h incubation period. Rabbit polyclonal antibodies directed to the rat hepatocyte integrin beta 1-chain inhibited the PDGF-stimulated collagen gel contraction. The inhibitory activity on contraction of the anti-beta 1 integrin IgG could be overcome by adding higher doses of PDGF. The contraction process was not blocked by anti-fibronectin IgG nor by synthetic peptides containing the tripeptide Arg-Gly-Asp (RGD), in concentrations that readily blocked fibroblast attachment to fibronectin-coated planar substrates. Autologous fibronectin or control peptides containing the tripeptide Arg-Gly-Glu were without effect. Immunofluorescence microscopy on fibroblasts grown within collagen gels revealed a punctate distribution of the beta 1 integrin and a lack of detectable levels of endogenously produced fibronectin. Collectively these data suggest a role for integrin collagen receptors with affinity for collagen fibers, distinct from the previously described RGD-dependent fibronectin receptors, in the fibronectin-independent PDGF-stimulated collagen gel contraction process.

Publiceringsår

1990

Språk

Engelska

Sidor

264-272

Publikation/Tidskrift/Serie

Experimental Cell Research

Volym

186

Issue

2

Dokumenttyp

Artikel i tidskrift

Förlag

Academic Press

Ämne

  • Psychiatry

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1090-2422