Analysis of methylhexahydrophthalic acid in human urine
Författare
Summary, in English
A method for analysis of urinary methylhexahydrophthalic acid (MHHP acid), a metabolite of the highly sensitising methylhexahydrophthalic anhydride, is described. The method is based on a double liquid-solid extraction of the MHHP acid from urine, esterification with methanol and boron trifluoride catalysis, and analysis with gas chromatography-mass spectrometry in the electron impact mode. The detection limit was 3 ng mL–1 and the precision between 2 and 5% in the range 34 to 340 ng mL–1. The method gave similar results compared to another method. Urine stored for five years at –20°C lost between 38 and 86% of the MHHP acid (range 16–85 ng mL–1).
Avdelning/ar
Publiceringsår
1996
Språk
Engelska
Sidor
668-670
Publikation/Tidskrift/Serie
Chromatographia
Volym
43
Issue
11-12
Dokumenttyp
Artikel i tidskrift
Förlag
Vieweg Verlag
Ämne
- Environmental Health and Occupational Health
Nyckelord
- Industrial hygiene
- Biological monitoring
- Methylhexahydrophthalic acid
- Gas chromatography-mass spectrometry
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 0009-5893