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Prompt epithelial damage and restitution processes in allergen challenged guinea-pig trachea in vivo

In English

Författare

Summary, in English

BACKGROUND: Little is known about the induction and the morphology of epithelial damage, and of the ensuing epithelial restitution processes in allergic airways. OBJECTIVE: To examine epithelial damage and restitution in allergen challenged guinea-pig trachea. METHODS: Whole-mount techniques, transmission and scanning electron microscopy, cryosectioning, and histochemical staining were used. Cell proliferation was monitored by BrdU-immunohistochemistry. RESULTS: Allergen challenge produced patchy, crater-like, and leucocyte-rich epithelial damage sites. At 1, 5, and 24 h damage was associated with poorly differentiated epithelial restitution cells. Already at 1 h the epithelial craters had a floor of flattened restitution cells and the damaged areas comprised < 1% of the mucosal surface area (whole-mount preparations). In contrast, cryo sections displayed large areas (approximately 20%, 1 h) of denudation. Epithelial, and subepithelial (fibroblasts, smooth muscle) proliferation was increased 5 and 24 h after challenge (P < 0.01). CONCLUSION: Within 1 h allergen challenge has induced patchy damage sites where epithelial restitution is already advanced; although easily produced by cryosectioning frank denudation was not evident in whole-mount preparations. The present findings may explain the well maintained, functional tightness of allergic airways displaying epithelial damage, shedding, and even denudation. The present data also suggest the possibility that epithelial damage-restitution may be causative to allergic airway remodelling.

Publiceringsår

1997

Språk

Engelska

Sidor

1458-1470

Publikation/Tidskrift/Serie

Clinical and Experimental Allergy

Volym

27

Issue

12

Dokumenttyp

Artikel i tidskrift

Förlag

Wiley

Ämne

  • Respiratory Medicine and Allergy

Nyckelord

  • epithelial restitution
  • allergic airways
  • epithelial damage

Status

Published

Forskningsgrupp

  • Airway Inflammation and Immunology

ISBN/ISSN/Övrigt

  • ISSN: 1365-2222