Differential usage of IκBα and IκBβ in regulation of apoptosis versus gene expression

Summary, in English

n this study we use the N-substituted benzamides declopramide (3-CPA) and N-acetyl declopramide (Na-3-CPA) to investigate the involvement of the transcription factor NF-κB in the induction of apoptosis and surface immunoglobulin κ (Igκ) expression in the mouse pre-B cell line 70Z/3. We first showed that 3-CPA-induced apoptosis at doses around 500 μM and that the 3-CPA-induced apoptosis could be suppressed by over-expression of the Bcl-2 protein. Na-3-CPA was shown to be non-apoptotic at doses up to 1–2 mM. On the other hand, Na-3-CPA inhibited LPS-induced Igκ expression while 3-CPA had no effect. Further analysis showed that while 3-CPA inhibited breakdown of IκBα, Na-3-CPA inhibited breakdown of IκBβ. In addition, we used a 70Z/3 cell line expressing a dominant negative IκBα (70Z/3ΔNIκBα). The 70Z/3ΔNIκBα cell line was shown to be more sensitive to apoptosis and cytotoxicity induced by 3-CPA as well as by LPS, probably due to a defect in NF-κB rescue mechanism. Taken together, our data implicate distinct roles for IκBα and IκBβ in regulating various NF-κB activities.

• Författare: Hanna Lindgren; Anders Olsson; Ronald Pero; Tomas Leanderson
• Avdelning/ar: Immunology
• Publiceringsår: 2003
• Språk: Engelska
• Sidor: 204-211
• Publikation/Tidskrift/Serie: Biochemical and Biophysical Research Communications
• Volym: 301
• Issue: 1
• Länkar: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12535663&dopt=Abstract; http://dx.doi.org/10.1016/S0006-291X(02)03012-7
• Dokumenttyp: Artikel i tidskrift
• Förlag: Elsevier
• Ämne: Biological Sciences
• Nyckelord: NF-κB; Apoptosis; IκB; N-substituted benzamides
• Status: Published
• Forskningsgrupp: Immunology
• ISBN/ISSN/Övrigt: ISSN: 1090-2104