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Ultrastructural characterization of dissociated embryonic ventral mesencephalic tissue treated with neuroprotectants.

In English

Författare

  • Young Hwan Ahn
  • Mia Emgård-Mattson
  • Patrik Brundin

Summary, in English

Poor survival and differentiation of grafted dopamine neurons limits the application of clinical transplantation in Parkinson’s disease. The survival of grafted dopamine neurons is only improved by a factor of 2–3 by adding neuroprotectants during tissue preparation. We used dye exclusion cell viability and electron microscopy to investigate the effects of the caspase inhibitor ac-YVAD-cmk and the lazaroid tirilazad mesylate on ultrastructural changes in dissociated embryonic mesencephalic cells. In addition, we examined whether the neuroprotectants selectively counteracted specific signs of neurodegeneration. Cell viability decreased significantly over time in both control and treated cell suspensions, but the number of viable cells remaining was significantly higher in tirilazad mesylate-treated cell suspensions. In control samples, the proportion of cells with an ultrastructure consistent with healthy cells decreased from 70%, immediately after dissociation, to 30% after 8 h of incubation. Similar changes were also observed in cell suspensions treated with neuroprotectants. Thus, the neuroprotectants examined did not block the development of specific morphological signs of neurodegeneration. However, when also taking into account that dead cells lysed and disappeared from each cell suspension with time, we found that the total number of remaining viable cells with healthy nuclear chromatin or intact membrane integrity was significantly higher in the tirilazad mesylate-treated group. The results indicate that tirilazad mesylate protects only a small subpopulation of embryonic mesencephalic cells from degeneration induced by mechanical trauma during tissue dissection and dissociation.

Publiceringsår

2003

Språk

Engelska

Sidor

235-241

Publikation/Tidskrift/Serie

Cell Transplantation

Volym

12

Issue

3

Dokumenttyp

Artikel i tidskrift

Förlag

Cognizant Communication Corporation

Ämne

  • Neurosciences

Nyckelord

  • Embryonic mesencephalic
  • Ultrastructure
  • Lazaroid
  • Caspase inhibitor
  • Viability
  • Survival

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1555-3892