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Degradation of Pyrimidines in Saccharomyces Kluyveri: Transamination of beta-Alanine.

Författare:
Publiceringsår: 2008
Språk: Engelska
Sidor: 794-799
Publikation/Tidskrift/Serie: Nucleosides, nucleotides & nucleic acids
Volym: 27
Nummer: 6
Dokumenttyp: Artikel
Förlag: Taylor and Francis Ltd

Sammanfattning

Beta-alanine is an intermediate in the reductive degradation of uracil. Recently we have identified and characterized the Saccharomyces kluyveri PYD4 gene and the corresponding enzyme beta -alanine aminotransferase ((Sk)Pyd4p), highly homologous to eukaryotic gamma-aminobutyrate aminotransferase (GABA-AT). S. kluyveri has two aminotransferases, GABA aminotransferase ((Sk)Uga1p) with 80% and (Sk)Pyd4p with 55% identity to S. cerevisiae GABA-AT. (Sk)Pyd4p is a typical pyridoxal phosphate-dependent aminotransferase, specific for alpha -ketoglutarate (alpha KG), beta -alanine (BAL) and gamma -aminobutyrate (GABA), showing a ping-pong kinetic mechanism involving two half-reactions and substrate inhibition. (Sk)Uga1p accepts only alpha KG and GABA but not BAL, thus only (Sk)Pydy4p belongs to the uracil degradative pathway.

Disputation

Nyckelord

  • Biology and Life Sciences
  • Saccharomyces kluyveri
  • Aminotransferase
  • gene duplication
  • γ-aminobutyrate
  • β-alanine

Övrigt

Published
Yes
  • ISSN: 1525-7770

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