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Exploring the context of the lung proteome within the airway mucosa following allergen challenge.

Författare:
  • Thomas E Fehniger
  • José-Gabriel Sato-Folatre
  • Johan Malmström
  • Magnus Berglund
  • Claes Lindberg
  • Charlotte Brange
  • Henrik Lindberg
  • György Marko-Varga
Publiceringsår: 2004
Språk: Engelska
Sidor: 307-320
Publikation/Tidskrift/Serie: J Proteome Res
Volym: 3
Nummer: 2
Dokumenttyp: Artikel
Förlag: American Chemical Society

Sammanfattning

The lung proteome is a dynamic collection of specialized proteins related to pulmonary function. Many cells of different derivations, activation states, and levels of maturity contribute to the changing environment, which produces the lung proteome. Inflammatory cells reacting to environmental challenge, for example from allergens, produce and secrete proteins which have profound effects on both resident and nonresident cells located in airways, alveoli, and the vascular tree which provides blood cells to the parenchyma alveolar bed for gas exchange. In an experimental model of allergic airway inflammation, we have compared control and allergen challenged lung compartments to determine global protein expression patterns using 2D-gel electrophoresis and subsequent spot identification by MS/MS mass spectrometry. We have then specifically isolated the epithelial mucosal layer, which lines conducting airways, from control and allergen challenged lungs, using laser capture technology and performed proteome identification on these selected cell samples. A central component of our investigations has been to contextually relate the histological features of the dynamic pulmonary environment to the changes in protein expression observed following challenge. Our results provide new information of the complexity of the submucosa/epithelium interface and the mechanisms behind the transformation of airway epithelium from normal steady states to functionally activated states.

Disputation

Nyckelord

  • Biology and Life Sciences
  • Medicine and Health Sciences
  • lung
  • mucosa
  • epithelium
  • laser capture microscopy
  • proteome mass spectrometry
  • annotation identity
  • allergen

Övriga

Published
Yes
  • ISSN: 1535-3893

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