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Acoustic microfluidic chip technology to facilitate automation of phage display selection

In English

Författare

Summary, in English

Modern tools in proteomics require access to large arrays of specific binders for use in multiplex array formats, such as microarrays, to decipher complex biological processes. Combinatorial protein libraries offer a solution to the generation of collections of specific binders, but unit operations in the process to isolate binders from such libraries must be automatable to ensure an efficient procedure. In the present study, we show how a microfluidic concept that utilizes particle separation in an acoustic force field can be used to efficiently separate antigen-bound from unbound members of such libraries in a continuous flow format. Such a technology has the hallmarks for incorporation in a fully automated selection system for the isolation of specific binders.

Publiceringsår

2008

Språk

Engelska

Sidor

5657-5666

Publikation/Tidskrift/Serie

The FEBS Journal

Volym

275

Issue

22

Dokumenttyp

Artikel i tidskrift

Förlag

Wiley-Blackwell

Ämne

  • Biochemistry and Molecular Biology

Status

Published

Projekt

  • Human IgE repertoires and an anti-allergome resource

ISBN/ISSN/Övrigt

  • ISSN: 1742-464X