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Characterization of water-soluble hemicelluloses from spruce and aspen employing SEC/MALDI mass spectroscopy

Författare:
Publiceringsår: 2002
Språk: Engelska
Sidor: 711-717
Publikation/Tidskrift/Serie: Carbohydrate Research
Volym: 337
Nummer: 8
Dokumenttyp: Artikel
Förlag: Elsevier

Sammanfattning

Partly depolymerized hemicelluloses isolated from wood chips of spruce and aspen employing microwave treatment were resolved using size-exclusion chromatography (SEC) into oligo- and polysaccharide fractions containing components with a narrow range of sizes, as determined by MALDI mass spectroscopy. The degree of substitution with acetyl moieties (DS) was also calculated on the basis of the MALDI-MS spectra obtained prior to and following deacetylation. For spruce hemicelluloses, the low molecular mass fraction contained small arabino-4-O-methylglucuronoxylan oligosaccharides, with DP values ranging from 4 to ~20, separated primarily on the basis of their charge density. The fraction eluted last consisted of an O-acetyl-(galacto)glucomannan polysaccharide of peak-average DP value (DPp) 14. The degree of substitution with acetyl groups (DS) decreased with decreasing DP, a value DS of 0.39 being obtained for the fraction with DPp 12. For the aspen hemicelluloses, the SEC fractions eluted first contained an acidic O-acetyl-4-O-methylglucuronoxylan polysaccharide with DP ranging from 10 to ~28 and an average DS of ~0.75. The fractions eluted last consisted of oligosaccharide mixtures composed primarily of small neutral O-acetyl-xylooligosaccharides (DPp 6, DS 0.41), together with minor quantities of an O-acetyl-glucomannan.

Disputation

Nyckelord

  • Chemistry
  • Aspen wood
  • Spruce wood
  • Hemicellulose
  • O-Acetylation
  • Mannan
  • Xylan
  • Size-exclusion Chromatography
  • Microwave treatment
  • MALDI-TOF-MS

Övriga

Published
Yes
  • ISSN: 0008-6215

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