Du är här

Mannan-Degrading Enzymes from Cellulomonas fimi

Författare:
Publiceringsår: 1999
Språk: Engelska
Sidor: 2598-2605
Publikation/Tidskrift/Serie: Applied and Environmental Microbiology
Volym: 65
Nummer: 6
Dokumenttyp: Artikel
Förlag: American Society for Microbiology

Sammanfattning

The genes man26a and man2A from Cellulomonas fimi encode mannanase 26A (Man26A) and -mannosidase 2A (Man2A), respectively. Mature Man26A is a secreted, modular protein of 951 amino acids, comprising a catalytic module in family 26 of glycosyl hydrolases, an S-layer homology module, and two modules of unknown function. Exposure of Man26A produced by Escherichia coli to C. fimi protease generates active fragments of the enzyme that correspond to polypeptides with mannanase activity produced by C. fimi during growth on mannans, indicating that it may be the only mannanase produced by the organism. A significant fraction of the Man26A produced by C. fimi remains cell associated. Man2A is an intracellular enzyme comprising a catalytic module in a subfamily of family 2 of the glycosyl hydrolases that at present contains only mammalian -mannosidases.

Disputation

Nyckelord

  • Chemistry

Övriga

Published
Yes
  • ISSN: 0099-2240

Box 117, 221 00 LUND
Telefon 046-222 00 00 (växel)
Telefax 046-222 47 20
lu [at] lu [dot] se

LERU logotype U21 logotype

Fakturaadress: Box 188, 221 00 LUND
Organisationsnummer: 202100-3211
Om webbplatsen