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Analysis of Molecular Size Distributions of Cellulose Molecules during Hydrolysis of Cellulose by Recombinant Cellulomonas fimi -1,4-Glucanases

In English

Författare

  • Henrik Stålbrand
  • Shawn D Mansfield
  • John N Saddler
  • Douglas G Kilburn
  • R Antony J Warren
  • Neil R Gilkes

Summary, in English

Four -1,4-glucanases (cellulases) of the cellulolytic bacterium Cellulomonas fimi were purified from Escherichia coli cells transformed with recombinant plasmids. Previous analyses using soluble substrates had suggested that CenA and CenC were endoglucanases while CbhA and CbhB resembled the exo-acting cellobiohydrolases produced by cellulolytic fungi. Analysis of molecular size distributions during cellulose hydrolysis by the individual enzymes confirmed these preliminary findings and provided further evidence that endoglucanase CenC has a more processive hydrolytic activity than CenA. The significant differences between the size distributions obtained during hydrolysis of bacterial microcrystalline cellulose and acid-swollen cellulose can be explained in terms of the accessibility of -1,4-glucan chains to enzyme attack. Endoglucanases and cellobiohydrolases were much more easily distinguished when the acid-swollen substrate was used.

Publiceringsår

1998

Språk

Engelska

Sidor

2374-2379

Publikation/Tidskrift/Serie

Applied and Environmental Microbiology

Volym

64

Issue

7

Dokumenttyp

Artikel i tidskrift

Förlag

American Society for Microbiology

Ämne

  • Biological Sciences

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 0099-2240